Previously, a monoclonal antibody was developed to the lysergic ring common to ergoline alkaloids (EA). The objectives were to develop an immunoassay for EA in tall fescue by determining (i) time requirement; (ii) tissue mass, and (iii) optimum volume of extraction solution necessary for detection protocols with repeatable results; and (iv) the acceptability of oven‐ versus freeze‐dried samples. A competitive enzyme‐linked immunosorbent assay (ELISA) was conducted on plant extracts (0‐548 μg kg−1 ergovaline). Regressions of ELISA and high performance liquid chromatography (HPLC) data gave good fits (R2 ≥ 0.87, MSE ≤ 0.03). Using 0.10 g (±0.01 g) of oven‐dried tissue, and extraction for 15 min in a 1:40 dilution, we analyze 200 samples daily.
No takes yet. Share an insight, caveat, or question.
Hill et al. (1994) studied this question.