It is shown that bone fluorescence reduction by bleaching in 30% hydrogen peroxide causes a change in the mineral/matrix ratio as measured by Raman spectroscopy. However, there are no observable spectral shifts in the mineral Raman spectrum or in the matrix Raman spectrum. Possible sources of the change in ratio are discussed. Peroxide bleaching is not recommended for fluorescence reduction in quantitative Raman spectroscopy.
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Chen et al. (2002) studied this question.
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