Four Permanent Cell Lines Established from Human Malignant Gliomas

Twenty-two human gliomas were set up in tissue culture and inoculated into nude mice. Permanent cell lines were established from four of these and their growth characteristics, and morphological features defined and cytogenetic features described. All four failed to sustain evidence of glial differentiation while three of the four cell lines exhibited the characteristics of striated muscle, and were tumorigenic in nude mice. Nine gliomas showed some initial growth in nude mice but only two were successfully subpassaged.