A rapid high-performance liquid chromatographic procedure for separation of the triacylglycerols (TG) of animal fats using refractive index detection is described. A LiChrospher-100 RP-18 (5 μm) column was used for the TG-profiling of pork, beef, mutton, chicken and turkey fats. Detection of pork fat in processed foods and lard in fat-admixtures is also discussed. TG-separation and checking genuinity and adulteration was achieved isocratically in ∼ 15 min. by using CH3CN/CH2Cl2 (58:42, v/v) at ambient temperature.
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Al-Rashood et al. (1995) studied this question.
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