PLCknockdown effectively decrease the MNU induced rat bladder tumorigenesis through a H-ras pathway

The crucial role of phospholipase C epsilon (PLC ε) in cancinogensis has been described recently. However, whether or not it decreases the methyl-nitroso-urea (MNU) induced rats’ bladder tumorigenesis and the probable mechanism remains poorly understood. Here, we investigated the efficacy of PLCε knockdown to MNU induced bladder tumor and its possible mechanism. First, a plasmid contains small hairpin Ribonucleic acid (RNA) against PLC ε was constructed. Then, this plasmid were intravesically installed into SD rats to silence the expression of PLC ε. 40 PLC ε silenced SD rats and 40 normal rats were intravesically installed with MNU, respectively. Another 10 normal SD rats were intravesically installed with saline. 12 weeks after installation, all rats were sacrificed. The bladders were harvested for pathological and histological examination, Reverse transcription polymerase chain reaction (RT-PCR) and Western-blotting. The pathological and histological examination results showed that 35 rats appeared tumor in normal rats group, while no rats appeared tumor in PLC ε silenced group and saline group. RT-PCR and Western-blotting showed PLC ε , PCNA were overexpressed in 35 tumor appeared rats and lowly expressed in other rats. The results reveal that the PLCε knockdown could interrupt the signal transduction in order to prevent MNU induced rats bladder tumorigenesis.