Pullulan was used as a sieving medium in high-performance capillary gel electrophoresis to study the effect of temperature and viscosity on electrophoretic behavior of sodium dodecyl sulfate (SDS)-proteins with molecular masses in the range of 14,400-116,000 Da. The migration time decreased with increasing capillary temperature. Equations were derived relating mobility of SDS-proteins to capillary temperature and viscosity of pullulan solution. Linear relationships were obtained from experiments between logarithm of mobility and reciprocal temperature and between double logarithmic values of mobility and viscosity of pullulan solution. The experimental results agree well with the equations.
No takes yet. Share an insight, caveat, or question.
Nakatani et al. (1996) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: