KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin

Figures

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  Figure 1.. KATP subunit expression and subcellular localization. (A) The mRNA expression of KATP subunit, including Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B, was measured by quantitative real-time PCR. (B) The subcellular localization of KATP subunit SUR was measured by BODIPY-glibenclamide and 5-µM DiIC18(3). KATP, ATP-sensitive potassium.

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  Figure 2.. High glucose promotes mesangial cell proliferation and production of MMP-2 and fibronectin. Rat mesangial cells were cultured with different doses of high glucose (HG, 10, 20, 30 or 40 mM) for 12 or 24 h. (A and B) The cell proliferation was measured by CCK-8 assay. (C and D) The production of MMP-2 and fibronectin was measured by ELISA. **P<0.01 compared with the control. MMP, matrix metalloproteinase.

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  Figure 3.. DZX inhibits HG-induced KATP subunit expression. Rat mesangial cells were cultured with 30 mM HG for 24 h. (A) The mRNA expression of KATP subunit, including Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B, was measured by quantitative real-time PCR. Rat mesangial cells were cultured with DZX or/and 5-HD prior to 30 mM HG for 24 h. **P<0.01 compared with the control. (B) The mRNA expression of KATP subunit, including Kir6.1, SUR1, SUR2A and SUR2B, was measured by quantitative real-time PCR. *P<0.05, **P<0.01 compared with HG; ##P<0.01 compared with HG+DZX. DZX, diazoxide; KATP, ATP-sensitive potassium.

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  Figure 4.. DZX inhibits HG-induced mesangial cell proliferation and production of MMP-2 and fibronectin. Rat mesangial cells were cultured with DZX or/and 5-HD prior to 30 mM HG for 12 or 24 h. (A and B) The cell proliferation was measured by CCK-8 assay. (C) The production of MMP-2 and fibronectin was measured by ELISA. **P<0.01 compared with HG; ##P<0.01 compared with HG+DZX. DZX, diazoxide; MMP, matrix metalloproteinase.