What does this research mean for the field?

Opposite changes in cardiac hemodynamic workload (hypertrophy and atrophy) induce a concordant microRNA expression signature that is distinct from the fetal microRNA program. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

December 9, 2010PLoS ONEOpen Access

Common MicroRNA Signatures in Cardiac Hypertrophic and Atrophic Remodeling Induced by Changes in Hemodynamic Load

Q: What is the clinical evidence from this study?

Population: Cardiac hypertrophy and atrophy (n=33). Intervention: Changes in hemodynamic load (ascending aortic stenosis and heterotopic heart transplantation) vs. Sham-operated and native hearts. Primary outcome: miRNA expression pattern.

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Key result

Opposite cardiac workload changes induce highly concordant miRNA expression patterns, divergent from fetal programs.

n=33

Why the study?

Mechanical overload and unloading induce cardiac hypertrophy and atrophy with similar transcriptional changes, but the role of miRNAs in determining cardiomyocyte plasticity direction is unclear.

Comparison

Ascending aortic stenosis-induced overload vs heterotopic heart transplantation-induced unloading vs sham and healthy controls

Design

Preclinical experimental study using quantitative stem-loop specific RT-PCR of miRNAs

Authors

AEAli El‐ArmoucheElectrophysiology ASAlexander Peter SchwoererUniversität Hamburg CNChristiane NeuberUniversität Hamburg

Discussion

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Implication

Challenges fetal miRNA program model in adult remodeling; hypothesis-generating for workload-specific miRNA targets in animals.

Structured PICO

Population

33 male Lewis rats subjected to ascending aortic stenosis or heterotopic heart transplantation to induce cardiac hypertrophy or atrophy, compared to sham and native controls.

Intervention

Ascending aortic stenosis (AS) to induce mechanical overloading, and heterotopic heart transplantation (HTX) to induce mechanical unloading

Comparator

Sham-operated and healthy controls

Outcome

miRNA expression patterns (panel of 351 miRNAs)surrogate

Opposite changes in cardiac workload (hypertrophy vs atrophy) induce a common miRNA expression pattern that is distinct from the fetal program.

Limitations

Expression of miRNAs was analyzed using primers based on human mature miRNA sequences, potentially missing rat-specific miRNAs.
The duration of overloading (7 days) and unloading (14 days) may not correspond to the time at which changes in miRNA expression have their major impact.
Target prediction analysis did not reveal specific sets of genes involved in defined cell-biological aspects.
Does not exclude that remodeling may be controlled by fine-tuning within a large network involving minimal changes in many individual miRNAs.

Cite This Study

El‐Armouche et al. (2010) studied Cardiac hypertrophy and atrophy (n=33). Changes in hemodynamic load (ascending aortic stenosis and heterotopic heart transplantation) vs. Sham-operated and native hearts was evaluated on miRNA expression pattern. Opposite changes in cardiac workload (hypertrophy and atrophy) induced a highly concordant miRNA expression pattern (r2=0.79) that did not resemble the fetal miRNA program.

synapsesocial.com/papers/6ab9d70c2dc0fb7efdc04c12 https://doi.org/10.1371/journal.pone.0014263

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Common MicroRNA Signatures in Cardiac Hypertrophic and Atrophic Remodeling Induced by Changes in Hemodynamic Load

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Common MicroRNA Signatures in Cardiac Hypertrophic and Atrophic Remodeling Induced by Changes in Hemodynamic Load

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

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