Egg White-Milk Mixture as a Novel Biological Storage Media for Avulsed teeth Periodontal Ligament Cells: An In-Vitro Study

Purpose: This study aimed to evaluate egg white-milk mixture as a novel storage medium for avulsed teeth compared to Hank's Balanced Salt Solution (HBSS) media. Materials and media:Ninety incisors teeth of 23 New Zealand rabbits were atraumatically extracted and divided into 6 equal groups.Group 1, 2 & 3 were immediately stored in HBSS media for 1, 4 & 6h respectively, while group 4, 5 & 6 were stored in egg white-milk for 1, 4 & 6h respectively.The teeth were prepared for histological investigation and digital counting of periodontal ligament (PDL) cells using trypan blue exclusion technique.Results: Teeth stored in HBSS showed that 65.75%, 48.25% & 38.08% of PDL cells were vital after 1, 4 & 6h storage time respectively, while egg white-milk reported 49%, 50.06% & 47.67% for the same storage times.In comparing, there is highly significant difference in favour of HBSS after1h storage time.However, this difference is converted in favour of the novel mixture with insignificant difference after 4h and high significant difference after 6h.Conclusion: Egg white-Milk mixture has the ability of replenishment of metabolites in depleted cells.Moreover it has significant superior properties than the approved evidences of HBSS especially for teeth stored for more than 4h.