Key result
AAV-mediated restoration of CYP7B1 expression reduced the expression of inflammation- and fibrosis-related genes in mice fed a methionine choline-deficient diet.
Restoration of CYP7B1 expression attenuates hepatitis and fibrosis in a mouse model of NASH, highlighting its potential role in disease progression.
Cyp7b1 downregulation may contribute to NASH progression in mouse models; hypothesis-generating for human therapeutic targeting.
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide, with an incidence of >25% of the adult population. NAFLD ranges from benign simple steatosis to non-alcoholic steatohepatitis (NASH). However, its transition mechanisms underlying the pathogenesis remain to be clarified. The expression of Cyp7b1 gene is downregulated in the liver of leptin-deficient mice and methionine and choline-deficient diet-fed mice based on previous microarray data. Thus, in this study, we investigated the effect of CYP7B1 restoration on the progression of NASH in mice fed MCD diet and its association with oxidative and lipid stresses. Our results suggest that restoration of CYP7B1 expression attenuates hepatitis and fibrosis and that lipid and oxidative stresses observed in the early stage of NASH suppresses Cyp7b1 transcription in hepatocytes.
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Watanabe et al. (2023) studied Non-alcoholic steatohepatitis (NASH). AAV-mediated restoration of CYP7B1 vs. AAV2/8-EGFP (mock) was evaluated on Expression of inflammation- and fibrosis-related genes. AAV-mediated restoration of CYP7B1 expression reduced the expression of inflammation- and fibrosis-related genes in mice fed a methionine choline-deficient diet.
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