Gravitational force is one of main forces necessary for separation of particles in gravitational field-flow fractionation. It is proportional to particle size and density. The influence of density on the elution behavior was studied using zeolites containing varying amounts of platinum. The other properties of the zeolite samples (e.g., size, shape, particle size distribution) were the same as those of the original zeolite sample without platinum. We proved that retention of particles was related to their densities. However, densities could not be calculated from elution data because we found that zeolites were eluted in a focusing elution mode. This mode was induced by hydrodynamic lift-forces and their nature has not been fully understood yet; this excludes exact calculation of density. The effects of sample preparation, amounts of sample injected, and flow rates of a carrier liquid are described.
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Urbánková et al. (1997) studied this question.
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