A collagenolytic enzyme was extracted from the 6000 g sediment of homogenized postmortal human colon tissue. The enzyme digests native collagen at neutral pH. The enzyme has been concentrated by ammonium sulfate precipitation. The collagenolytic activity was measured using [1-14C]acetylated calf skin collagen, solubilized in 0.1 g/1 acetic acid. There was a significant inactivation of the enzyme solution after the addition of mumol EDTA to the test volume.
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Kortmann et al. (1977) studied this question.
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