Key result
Early severe stroke mortality links to higher day-1 CSF IL-6 and larger MRI infarcts.
Why the study?
The study aimed to identify time-related changes in clinical, MRI, histopathologic, and immunohistochemical findings in a canine ischemic stroke model, and to assess the association of cerebrospinal fluid and tissue IL-6 levels with clinical prognosis.
Does permanent middle cerebral artery occlusion induce time-related changes in MRI lesion volume and cerebrospinal fluid and tissue IL-6 levels that correlate with neurological prognosis in a canine model of ischemic stroke?
Does permanent middle cerebral artery occlusion induce time-related changes in MRI lesion volume and cerebrospinal fluid and tissue IL-6 levels that correlate with neurological prognosis in a canine model of ischemic stroke?
Answers are grounded in this paper and cite their sources.
In a canine model of permanent middle cerebral artery occlusion, elevated day-1 cerebrospinal fluid IL-6 levels and larger MRI ischemic lesion volumes were strongly associated with acute mortality and severe neurological deterioration, supporting CSF IL-6 as a potential prognostic biomarker in acute ischemic stroke.
The purpose of this study was to identify time-related changes in clinical, MRI, histopathologic, and immunohistochemical findings associated with ischemic stroke in dogs. Additionally, the association of cerebrospinal fluid (CSF) and tissue levels of interleukin (IL)-6 with clinical prognosis was assessed. Ischemic stroke was induced by permanent middle cerebral artery occlusion (MCAO) in nine healthy experimental dogs. The dogs were divided into three groups according to survival time and duration of the experimental period: group A (survived only 1 day), group B (1-week experimental period), and group C (2-week experimental period). Neurologic status was evaluated daily. Magnetic resonance imaging (MRI) was performed according to a predetermined schedule. Concentration of IL-6 in CSF was measured serially after ischemic stroke. Postmortem examination was performed for all experimental dogs. During histopathological examination, variable degrees of cavitation and necrosis due to neuronal cytopathic effects, such as pyknotic nuclei and cytoplasmic shrinkage, were observed on the affected side of the cerebral cortex in all dogs. Immunohistochemistry specific for IL-6 showed increased expression in the ischemic lesions. CSF IL-6 concentrations and ischemic lesion volumes 1 day after ischemic stroke were significantly higher in group A compared to groups B and C.
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Jeon et al. (2015) studied Experimentally induced ischemic stroke via permanent middle cerebral artery occlusion (MCAO) (n=9). Permanent middle cerebral artery occlusion (MCAO) via artificial silicon embolus vs. Group A (survived 1 day, n=3) vs Group B (1-week survival, n=2) and Group C (2-week survival, n=4) was evaluated on Cerebrospinal fluid (CSF) IL-6 concentration and MRI ischemic lesion volume 1 day after ischemic stroke (p=<0.0016). Permanent middle cerebral artery occlusion in dogs resulted in significantly higher day-1 CSF IL-6 levels (p<0.0016) and MRI lesion volumes (p<0.0238) in dogs surviving 1 day versus 1-2 weeks.
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