The 4Na-EDTA-Giemsa staining of metaphase chromosomes from embryos of three small freshwater fish, zebrafish Danio rerio, medakafish Oryzias latipes, and rosy bitterling Rhodeus ocellatus, in the presence of BrdU for one cycle gave rise to clear bands along the length of the chromosomes. These bands (B-bands) with G-band-like structures were clear and reproducible. However, as distinct B-bands were observed only in elongated chromosomes, fine chromosome preparations with a high mitotic index and elongated chromosomes were required. A technique for making preparations from embryo cells satisfied this request. The B-banding technique applied to embryo cells is useful to analyze chromosomes of fish species in which ordinary G-banding techniques have been known to bring about only unsatisfactory results.
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Ueda et al. (1999) studied this question.
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