It has long been recognized that the elucidation of cestode metabolism, was first dependent upon the successful development of a method for culturing worms in vitro. The oligoseptic methods of Markov (1938) and bactericidal washings by Green and Wardle (1941) were only of a limited success. Recently both Smyth (1946), and Joyeux and Baer (1942) working independently, introduced a technique whereby plerocercoids of the Diphyllobothriidae could be removed and cultured under aseptic conditions.
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C. A. Hopkins (1950) studied this question.