Method Development and Validation for estimation of Moxifloxacin HCl in tablet dosage form by RP-HPLC method

Moxifloxacin HCl is chemically 1-cyclopropyl-7-((s,s)-2,8diazabicyclo(4.3.0)non-8-yl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3quinoline carboxylic acid.Moxifloxacin is an antibiotic used to treat respiratory infections, including acute sinusitis, acute exacerbations of chronic bronchitis, and community-acquired pneumonia, as well as dermatological infections, as a second-line agent in tuberculosis.The literature survey [1][2][3][4][5][6][7] reveals that there is some HPLC methods have been reported.In this paper we describe a simple, inexpensive, sensitive and validated HPLC [8-12] method for the determination of Moxifloxacin HCl in bulk and pharmaceutical formulation.Working standard of Moxifloxacin HCl was obtained from well reputed research laboratories.HPLC grade Methanol, Merck grade Orthophosphoric acid and Triethylamine and Milli-Q water were procured from the market.The separation was carried out on isocratic HPLC system with pre-packed Luna C-18, 5µ (250 x 4.6) mm column using filtered and degassed mixture of Buffer: Methanol (55:45) as mobile phase. Buffer PreparationTransferred 2 ml of orthophosphoric acid into 1000 ml of water and the P H were adjusted to 2.5 with triethylamine, filtered through 0.45µm nylon membrane filter and degassed. Mobile phaseBuffer and methanol were mixed in the ratio of 55:45 and sonicated to degas. Standard preparationAccurately Weighed and transferred Moxifloxacin HCl equivalent to 20 mg of Moxifloxacin Working Standard into a 50 ml clean dry volumetric flask, and 30 ml of mobile phase was added, sonicated for 5 minutes, and diluted to volume with mobile phase.Further diluted 5 ml to 50 ml with mobile phase.Flow rate 1.0 ml/min; detection wavelength 293 nm; injection volume 10µl; column used Luna C18 (5µm, 250x4.6 mm); column temperature: 25 o C; mobile phase: Buffer: Methanol (55:45).