Zusammensetzung der Phospholipide und deren Fettsäuremuster in Kaninchenerythrocyten und ghosts

The phospholipids of rabbit erythrocytes were separated by thin‐layer chromatography on silica gel HR and determined quantitatively by the molybdenum blue method. Prior to thinlayer chromatography phosphatidic acid and phosphatidyl inositol were concentrated by column chromatography. The total phospholipid contents were 313 ± 27 amoles1 per erythrocyte and 289 ± 13 amoles per ghost. The proportions of the individual phospholipids of erythrocytes in moles % were 36.3 of phosphatidyl choline, 32.6 of phosphatidyl ethanolamine, 15.7 of sphingomyelin, 12.4 of phosphatidyl serine, 1.6 of phosphatidic acid, 1.3 of phosphatidyl inositol, and < 0.1 of lysophosphatidyl choline. The same distribution was obtained in ghosts with the exception of a significantly higher concentration of sphingomyelin. The fatty acid composition of phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, ceramide trisaccharide, and sphingomyelin was analysed by gas‐liquid chromatography after the lipids had been fractionated by preparative thin‐layer chromatography. For identification of the fatty acids, their methylesters were isolated on silica gel G‐silver nitrate plates according to degree of saturation. Typical of sphingolipids were the low concentration of linoleic acid, the absence of arachidonic acid, the presence of lignoceric acid and the very high concentration of nervonic acid. Phosphatidyl choline and phosphatidyl ethanolamine were hydrolysed by phospholipase A (ophiophagus hannah) in order to analyse the fatty acid distribution of the two positions. In position 1 of phosphatidyl choline and phosphatidyl ethanolamine the saturated fatty acids amounted to 83.6% and 65.6%, respectively. Unsaturated fatty acids, especially oleic acid and linoleic acid, also occurred in position 1. These acids were the main components in position 2.