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The present communication reports a modified technique for staining myoepithelial cells in normal and neoplastic human salivary gland tissues in 2-3μm thick sections from epoxy blocks conventionally prepared for transmission electron microscopy. The method employs tannic acid-phosphomolybdic acid-levanol fast cyanine 5RN procedure by Puchtler et al. after intense oxidation of plastic-free sections using 5% potassium permanganate for 20min followed by bleaching in 1% oxalic acid for 20min. As for the resin-removal procedure, treatment with saturated solution of potassium hydroxide in absolute ethanol for 5-10min yields an optimum result. Direct correlations of light and electron microscopic observations indicate that the cells positively stained with levanol fast cyanine 5RN correspond well to the myoepithelial and neoplastic modified myoepithelial cells containing bundles of microfilaments. This approach is useful in matching the corresponding areas at the light and electron microscopic levels in a single block of tissue for evaluation of myoepithelial participation in various salivary gland tumors.
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EL-BARDAIE et al. (1986) studied this question.
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