Key result
SUT plus EAL rapidly clears mouse whole-hearts with only ~1% protein loss for 3D imaging.
Why the study?
A method to visualize cardiac microstructures and molecular changes in intact cardiac tissue with single-cell resolution was needed to explore mechanisms underlying cardiac diseases.
SUT advances cardiac microstructure imaging tools; leaves open clinical translation pending validation in disease models.
We developed a new method, SUT (Scheme Update on tissue Transparency), to view cardiac microstructures and unveil the molecular changes underlying cardiac diseases. SUT is an effective method to clear whole-hearts from different species. Over the course of 4 - 6 days we obtained transparent whole-layer left ventricular tissues from mice with only an approximate 1% protein loss. In addition, EAL (Electrophoretic Antibody Labeling) was used to achieve fast antibody labeling by electric force, which significantly reduced antibody incubation time from days to hours. SUT, together with EAL and modern imaging techniques, were successfully used to visualize three-dimensional spatial distribution of various molecules in cardiac tissue. We also observed changes in the number and phenotypes of fibroblasts during post-myocardial infarction in a stereoscopic pattern. We believe that our technique opens a new avenue to explore the mechanisms underlying cardiac diseases.
No takes yet. Share an insight, caveat, or question.
Wang et al. (2018) studied cardiac diseases. SUT (Scheme Update on tissue Transparency) and EAL (Electrophoretic Antibody Labeling) was evaluated on tissue transparency and protein loss.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: