Lymphotoxin (LT) was found to be present in the same supernatants as antigen-stimulated guinea pig lymphocytes in which migration-inhibitory factor (MIF) was found. LT activity was determined by staining target cells with trypan blue, counting the cells present in the media and determining their viability, direct observation by time-lapse cinematography and measuring protein synthesis by surviving target cells. Like migration-inhibitory factor, the induction of LT release was antigen specific. LT was effective on a number of different target cell lines; however, young cultures of guinea pig embryo fibroblasts were resistant to it.
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Rosenberg et al. (1973) studied this question.
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