Nematicidal metabolites from the fungusPleurotus ferulae Lenzi

Pleurotus ferulae Lenzi, a species of edible fungus, was found to have nematicidal activity in experiments searching for nematicidal fungi. Three nematicidal metabolities cheimonophyllon E (compound 1), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (compound 2) and 5-hydroxymethyl-furancarbaldehyde (compound 3) were isolated based on bioassay-guided fractionation from the extracts of the fungusP. ferulae. Their structures were determined by spectroscopic data. All of them showed activities against nematodesBursaphelenchus xylophilus (Steiner et Buhrer) Nickle andPanagrellus redivivus (Linn.) Goodey. The median lethal concentrations (LC50) of compounds 1, 2 and 3 at 72 h were 70.8, 174.6, and 54.7 mg L−1 respectively againstB. xylophilus and were 125.6, 128.1, and 82.8 mg L−1 respectively againstP. redivivus. The three compounds were obtained fromP. ferulae for the first time.