ZnFe2O4 nanoparticle-modified screen-printed electrode for determination of serotonin in the presence of dopamine and tryptophan

Background and purpose: An easy, accurate, faultless, meteoric and inexpensive methodology has to be created for determining important compounds serotonin (SRT), dopamine (DPE) and tryptophan (TPT). Experimental approach: This work explored the use of ZnFe2O4 nanoparticles to construct a ZnFe2O4 nanoparticle-modified screen-printed electrode (ZFO/SPE) sensor for analysing the biomolecule SRT in the presence of DPE and TPT. The results show that ZnFe2O4 nanoparticles can greatly enhance electron-transfer kinetics and exhibit high electrocatalytic activity, making them a useful tool for biomolecule analysis. For detection, differential pulse and cyclic voltammetry have been used to analyse the electrochemical treatment of the analytes. Key results: Under optimal conditions, the calibration curve of current versus SRT concentration in the range of 0.06 to 400.0 µM is linear. The obtained limit of detection is 0.02 µM. By employing this sensing system in detecting SRT, DPE and TPT in human urine and pharmaceutical samples to examine their feasibility for practical applications. The recovery percentage analysis for the real sample was found to be in the range of 96.9 to 103.5. Conclusion: It can be concluded that the ZFO/SPE possesses the features of good stability, reproducibility and repeatability for the regular detection of SRT, DPE and TPT.