Key result
CK-MB and MLC-1 immunohistologic stains reliably identify myocardium, rhabdomyosarcomatous elements, and regenerating skeletal muscle.
Why the study?
Immunohistologic staining for myocardial proteins CK-MB and MLC-1 was investigated for potential applications as tumor and regenerative muscle fiber markers.
Answers are grounded in this paper and cite their sources.
The authors used antibodies specific for creatine kinase MB isoenzyme (CK-MB) and myosin light chain-1 (MLC-1) for immunohistologic staining. At appropriate dilutions of antibody, frozen sections of human heart muscle were positive for both CK-MB and MLC-1, whereas sections of human skeletal muscle were negative for both proteins. Staining for both CK-MB and MLC-1 also was demonstrated in an immature teratoma. Furthermore, staining was localized to the rhabdomyosarcomatous elements within the teratoma; other components of the tumor did not stain for CK-MB or MLC-1. Biopsies of skeletal muscle revealed that regenerative, but not intact normal or degenerating, fibers also contained CK-MB and MLC-1. Immunohistologic stains for CK-MB and MLC-1 may be useful as tumor markers and as markers for regenerative muscle fibers.
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Emancipator et al. (1992) studied Tissue characterization (heart muscle, teratoma, skeletal muscle). Immunohistologic staining for CK-MB and MLC-1 was evaluated on Staining positivity for CK-MB and MLC-1 proteins. Immunohistologic stains for CK-MB and MLC-1 positively identified human heart muscle, rhabdomyosarcomatous elements within a teratoma, and regenerative skeletal muscle fibers.
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