Sex Differences and Caffeine Impact in Adenosine-Induced Hyperemia

Background: Caffeine consumption before adenosine stress myocardial perfusion imaging (MPI) is known to affect the hemodynamic response and, thus, reduce the stress myocardial blood flow (MBF) and myocardial flow reserve (MFR) assessments. However, it is not clear if any gender-specific differences in the hemodynamic response following caffeine consumption exist. This study aimed to evaluate if such differences exist and, if so, their impact on MBF and MFR assessments. Methods: This study comprised 40 healthy volunteers (19 women). All volunteers underwent four serial rest/stress MPI sessions employing Rubidium-82; two sessions were acquired without controlled caffeine consumption, and two sessions following oral ingestion of either 100mg and 300mg caffeine or 200mg and 400mg caffeine. For the caffeine imaging sessions, caffeine was ingested orally 1hr before the MPI scan. Results: Increase in plasma caffeine concentration (PCC) (mg/l) following consumption of caffeine was larger in women (MPI session without caffeine vs. MPI session with caffeine: women = 0.3±0.2 vs. 5.4±5.1, men = 0.1±0.2 vs. 2.7±2.6, both p<0.001). Caffeine consumption led to reduced stress MBF and MFR assessments for men while no changes were reported for women (women (PCC<1mg/l vs PCC≥1mg/l): stress MBF = 3.3±0.6 vs. 3.0±0.8 ml/g/min, P = 0.07; MFR = 3.7±0.6 vs. 3.5±1.0, P = 0.35; Men (PCC<1mg/l vs PCC≥1mg/l): stress MBF= 2.7±0.7 vs. 2.1±1.0 ml/g/min, P = 0.005, MFR = 3.8±1.0 vs. 3.1±1.4, P = 0.018). Significant differences in the stress MBF were observed for the two genders (both p≤0.001), while similar MFR were reported (both p≥0.12). Conclusion: Associations between increases in PCC and reductions in stress MBF and MFR were observed for men, while women did not have the same hemodynamic response. Stress MBF was affected at lower plasma caffeine concentrations in men than women.