A rapid and simple way to make imprints of plant epidermal structure for microscopic study is described. Rhoplex AC-33, a viscous emulsion of acrylic polymers, is applied to the tissue, allowed to dry to a transparent film, then peeled off, and placed on a microscope slide. The film records excellent structural detail and is relatively permanent. Rhoplex is non-toxic to the plant, and repeated imprints can be made for studies of guard cell movement or of epidermal cell development. Photomicrographs of various epidermi are presented.
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Horanic et al. (1967) studied this question.
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