Isolierung, Auftrennung und Strukturaufklärung des blauen Bakterienpigments „Amylocyanin" aus Streptomyces coelicolor Müller / Isolation, Separation and Structure of the Blue Pigment „Amylocyanin“ from Streptomyces coelicolor Müller

The isolation and chromatographic separation of “Amylocyanin”, the blue pigment mixture of Streptomyces coelicolor is described. The main component is 5-(hydroxy-3-β-D-ribopyranosyl-pyrid-2-on-5-yl)-imino-3-β-D-ribopyranosyl-2,6-pyridindione (1), as is prooved by degradation reactions, model compounds and spectroscopic data. The nine minor components are α- and β-D-ribopyranosyl- and α- and β-ribofuranosyl derivatives of 1. Degradation with acetic acid/phenylhydrazine yields the isomere 3-α- and 3-β-D-ribopyranosyl- and 3-α- and 3-β-D-ribofuranosyl-5-phenylhydrazono-2,6-pyridindiones 2, 8, 9 and 10; ozonolysis of 1 liberates D-ribose.