Vaccinia virus‐mediated damage of murine ovaries and protection by virus‐expressed interleukin‐2

Vaccinia virus was shown to replicate in the ovaries of normal inbred mice and cause sufficient damage to ovarian follicles to decrease fertility of the mice. The mouse-adapted strain, VV-WR, had a greater affinity for growth in ovaries than the vaccine strains, VV-Elstree, VV-NYBH or VV-Copenhagen. Virus reached the ovaries after intravenous or intraperitoneal inoculation, but not after subcutaneous inoculation in the foot pad. Interleukin-2 (IL-2), when expressed by a recombinant vaccinia virus was able to decrease the titre of virus in the ovaries and prevent infertility. Both non-specific (natural killer cells) and antigen-specific mechanisms were active within the ovaries and may play a role in the IL-2-mediated clearance of vaccinia virus.