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A remote electrochemical biosensor for field monitoring of organophosphate nerve agents is described. The new sensor relies on the coupling of the effective biocatalytic action of organophosphorus hydrolase (OPH) with a submersible amperometric probe design. This combination results in a fast, sensitive, selective, and stable response at large sample-instrument distances. Such attractive performance is illustrated for direct measurements of micromolar levels of paraoxon and methyl parathion in untreated river water samples. Unlike multi-step inhibition biosensors, the remote OPH probe offers single-step direct measurements, and is thus highly suitable for the continuous monitoring task. Variables relevant to field operations are discussed, along with prospects for remote monitoring and early detection of nerve agents.
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Wang et al. (1999) studied this question.
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