Key result
Anti-CD90 antibody coating significantly increases mesenchymal stem cell capture on decellularized valve scaffolds.
Why the study?
The importance of cell adhesion to the scaffold in tissue-engineered heart valves remains to be determined.
p-value: p=<0.05
Antibody-coated scaffolds enhance MSC retention under shear in vitro; hypothesis-generating for tissue-engineered valves pending in vivo validation.
The importance of cell adhesion to the scaffold in the tissue-engineered heart valve remains to be determined. The current study examined the feasibility of conjugating antibody against CD90 to a decellularized porcine aortic valve scaffold and binding mesenchymal stem cells to that scaffold through interaction with a cell surface antigen. After decellularization, the porcine aortic valve was reacted with biotin, avidin, and biotinylated anti-rat CD90 antibody sequentially and inserted into a laminar flow system used to test the effect of laminar shear stress. Rat bone mesenchymal stem cells (BMSC) were injected and circulated in a flow system to study the ability of anti-CD90 antibody to trap and immobilize cells on the valve surface. The results demonstrated that anti-CD90 antibody on the valve surface remains bound, even under high shear conditions. Compared with the control valve (no antibody), the modified (antibody-coated) valve immobilized significantly more rat BMSC (p < 0.05). Thus, the avidin-biotin system can be used to attach anti-CD90 antibody to these valves, and the bound antibody can immobilize rat BMSC in a flow chamber, suggesting that antibody-modified scaffolds might be used to fabricate shear stress-resistant, tissue-engineered heart valves.
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Ye et al. (2008) studied Tissue-engineered heart valves. Anti-CD90 antibody coating vs. Control valve (no antibody) was evaluated on Immobilization of rat bone mesenchymal stem cells on the valve surface (p=<0.05). Anti-CD90 antibody-coated decellularized porcine aortic valve scaffolds immobilized significantly more rat mesenchymal stem cells compared to control valves under high shear conditions (p < 0.05).