A correct lamp/filter combination is essential in order to optimize the accuracy of chlorophyll measurements made with filter fluorometers. For example, a nonoptimum lamp in a Sequoia‐Turner 111A fluorometer can affect the acid ratio. The magnitude of the acid ratio factor dictates the size of the error propagation from calibration to chlorophyll determination. A low value of the acid ratio causes a greater uncertainty in the chlorophyll determination. As long as the acid ratio is ≥2.0, any error in the acid ratio, which is determined at the time of instrument calibration, is not amplified in the calculations of chlorophyll concentration.
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Baker et al. (1983) studied this question.
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