Key result
Estradiol-treated stem cell conditioned media reduces cardiac oxidative stress and tissue damage in diabetic rats.
Why the study?
Diabetes mellitus is associated with cardiovascular disorders caused by oxidative stress, inflammation, and apoptosis, and the therapeutic effects of stem cells in diabetes require further evaluation.
Does estradiol-treated mesenchymal stem cell conditioned medium improve cardiovascular injury and signaling pathways in a diabetic male rat model?
Does estradiol-treated mesenchymal stem cell conditioned medium improve cardiovascular injury and signaling pathways in a diabetic male rat model?
Conditioned medium from mesenchymal stem cells, particularly when pretreated with estradiol, shows potential for mitigating diabetic cardiomyopathy by modulating redox balance and the PI3K/Akt-Atrogin-1 pathway.
Should not change clinical practice; hypothesis-generating for estradiol-primed stem cell media in diabetic cardiomyopathy, warranting human studies.
Diabetes mellitus is a metabolic disorder caused by insulin resistance or lack of insulin secretion. This disease is associated with cardiovascular disorders caused by oxidative stress, inflammation, and apoptosis. Previously, the therapeutic effects of stem cells have been shown in diabetes. In the present study, the effects of substances secreted from mesenchymal stem cells extracted from bone marrow with or without treatment with estradiol were evaluated on some characteristics of the cardiovascular system in male diabetic rats. In this study, 22 male Wistar rats weighing 190±20 g were used. Two rats were used to extract the mesenchymal stem cells (MSCs) from bone marrow. Stem cells were exposed to 0 or 0.1 mM Estradiol, and then, the conditioned media (CM and ECM) of the cells were harvested. Diabetes was induced by intraperitoneal injection of nicotinamide (110 mg/kg) and then streptozotocin (65 mg/kg). Diabetic animals were divided into three subgroups intraperitoneally receiving saline, CM, or ECM (100 µl/rat) three times on days 7, 21, and 35 after the beginning of the study. On the fiftieth day, a part of the cardiac apex was transferred to -70 °C to evaluate the levels of malondialdehyde (MDA), total antioxidant capacity (TAC), and the expression of PI3K, Akt, and Atrogin-1 genes by the qRT-PCR method. The rest of the cardiac tissue was placed in formalin for histopathologic study. Advanced glycation end products (AGEs) were measured in serum. Diabetes significantly (p < 0.05) caused to decrease of TAC and to increase of MDA, PI3K, Akt, and Atrogin-1 mRNA expression in the heart, and the serum AGEs levels compared to control rats, and led to cardiac tissue damage. Administration of CM or ECM significantly (p < 0.05) modulated all indices except Akt expression levels. In conclusion, conditioned media of bone marrow mesenchymal stem cells, especially ECM, improved some cardiovascular disorders in diabetic rats.
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Aghasi et al. (2026) studied Nicotinamide/STZ-Induced Diabetic Cardiomyopathy (n=22). Estradiol-treated mesenchymal stem cells conditioned medium (ECM) or standard conditioned medium (CM) vs. Saline was evaluated on Levels of malondialdehyde (MDA), total antioxidant capacity (TAC), and the expression of PI3K, Akt, and Atrogin-1 genes in cardiac tissue (p=<0.05). Administration of conditioned media from bone marrow mesenchymal stem cells, especially those treated with estradiol, significantly modulated oxidative stress, PI3K/Akt/Atrogin-1 mRNA expression, and improved cardiac tissue damage in diabetic rats (p < 0.05).
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