Biomechanical examinations of cancellous bone concerning the influence of duration and temperature of cryopreservation

The influence of cryopreservation of cancellous bone at different temperatures and different storage periods was investigated for mechanical changes. Standardized cancellous cylinders (50 x 25 mm) were stored at -20 degrees C or -80 degrees C for 6 or 12 months, respectively, or for 2 years and then subjected to a screw pull-out test. No significant differences were found among the various storage temperatures and periods investigated. We conclude that different cryopreservation temperatures do not have any influence on the mechanical properties of cancellous bone. Also, storage duration up to 2 years has no effects on the mechanical strength of the bone.