Simultaneous Identification of Multiple Protein Targets by Using Complementary‐DNA Phage Display and a Natural‐Product‐Mimetic Probe

Putting on a good display: A novel selection protocol was devised for the simultaneous display cloning of three homologues of the FK506-binding protein (FKBP12, FKBP12.6, and FKBP13) by using AP1497 (see structure), a mimetic of FK506, and a T7 complementary-DNA phage-display library. A quantitative on-phage binding assay was also performed to evaluate the affinity of the isolated proteins.