Using subcutaneous solid tumor produced by U251-SP human glioma cells, we studied the in vivo transfection of the cells with the human β-interferon (HuIFN-β) gene by means of novel liposomes. Since the tumor usually forms a single or multiple cysts when the tumor becomes over 15mm in diameter, we injected liposomes entrapping the gene into the cyst, and found that HuIFN-β was secreted into the cystic fluid. It reached a level of approximately 450IU/ml 48h after the transfection and remained at the same level for 8 days. The growth of the tumor was completely inhibited when the injection was carried out every second day for 10 days. The tumor did not grow at all for 30 days.
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Yoshida et al. (1991) studied this question.