The comparative efficacy of several methods of vaccinating rainbow trout, Salmo gairdneri Richardson, with Yersinia ruckeri bacterins was evaluated. In general, the protective immunity was best by intraperitoneal injection followed by direct immersion, shower and spray, respectively. The bacterin was effective by all methods when diluted 1:20 or less, but there was a trend towards less efficacy with higher dilutions and shorter exposure time.
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JOHNSON et al. (1983) studied this question.
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