Development of conventional and quantitative PCR assays for the detection of Vibrio europaeus using a Species-Specific gene identified from the pangenome

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Vibriosis, specifically caused by Vibrio europaeus, significantly impacts bivalve aquaculture through severe mortalities mainly at early stages. Developing specific diagnostic tools has been hindered by a lack of reliable molecular targets. Using a pangenomic approach via our custom pipeline, PanGenAmp, we identified the gene ktrA as a highly specific marker. Novel conventional and quantitative PCR (qPCR) assays were developed based on ktrA, demonstrating high sensitivity and accuracy. These PCR tools fill a critical diagnostic gap, enabling early pathogen detection and improved biosecurity to mitigate the economic consequences of V. europaeus outbreaks in shellfish hatcheries.