Key result
Endothelial cell-conditioned medium enhances adrenocortical aldosterone release up to ~2.5-fold following stimulation.
Why the study?
Alterations in vasculature and renin-angiotensin-aldosterone system are consistent in metabolic syndrome, but the role of endothelial cell-mediated activation of aldosterone synthesis in human adrenocortical cells is unclear.
Answers are grounded in this paper and cite their sources.
Endothelial factors may sensitize aldosterone responses; hypothesis-generating for vascular-adrenal crosstalk in metabolic syndrome, needs in vivo confirmation.
Alterations in both vasculature and renin-angiotensin-aldosterone system are a consistent finding in the metabolic syndrome. Adrenal tissue is highly vascularized and encounters blood flow, exceeding by far the volume expected for its size. Endothelial cells in the adrenal vasculature are therefore a major cellular component of adrenocortical tissue. The aim of the study was to analyze the cellular interaction between endothelial and steroid producing cells, focusing on endothelial cell-factor-mediated activation of aldosterone synthesis. The interaction between human endothelial (HUVECs) cell-conditioned medium and human adrenocortical (NCI-H295R) cells IN VITRO induced a significant surge in aldosterone secretion. The endothelial cell-conditioned medium together with angiotensin II and forskolin also potentiated aldosterone release by 1.5-fold and 2.6-fold, respectively, while preincubation of NCI-H295R cells for 24 h with endothelial cell-conditioned medium enhanced and sensitized the response of NCI-H295R to subsequent angiotensin II and forskolin stimuli by 2.5-fold and 2.2-fold, respectively. The increase in aldosterone release after preincubation with endothelial cell-conditioned medium was sensitive to cycloheximide and KN-93. Cellular conditioning with endothelial-cell factors exerts a hitherto unknown paracrine regulation of aldosterone production in human adrenocortical cells. This interaction may contribute to altered basal aldosterone release and have a role in patients with hypertension.
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Ansurudeen et al. (2006) studied this question. Endothelial cell-conditioned medium vs. Control medium was evaluated on Aldosterone release. Preincubation of human adrenocortical cells with endothelial cell-conditioned medium enhanced and sensitized aldosterone release in response to subsequent angiotensin II and forskolin stimuli by 2.5-fold and 2.2-fold, respectively.
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