Integrated High‐Resolution Mass Spectrometry‐based Chemical Identification and Quantitative Analysis of Multiple Components With a Single Marker for Quality Evaluation of Dasiphora mandshurica (Maxim.) Juz

The medicinal and edible herb Dasiphora mandshurica (Maxim.) Juz. (DMMJ), traditionally consumed as herbal tea from high‐altitude regions (>3200 m), has shown promising applications in dermatological care and immunomodulation, but its development is hindered by insufficient chemical characterization and quality standards. This study aimed to establish a comprehensive quality evaluation framework for DMMJ by integrating high‐resolution mass spectrometry‐based compound identification with quantitative analysis of multiple components using a single marker (QAMS), complemented by chemometric modeling. A total of 43 constituents were tentatively characterized, and chromatographic fingerprint analysis revealed pronounced compositional variability among samples collected from different altitudes, with specimens originating above 3200 m exhibiting fingerprint similarity exceeding 0.90. Comparative quantitative analysis demonstrated that there was no statistically significant difference ( p > 0.05) between QAMS and external standard method results, indicating the stability and reliability of the relative correction factors. Hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis were employed to elucidate altitude‐dependent chemical variations and to screen for potential discriminatory markers, indicating tiliroside and narcissoside as promising candidate markers for quality assessment of DMMJ. Collectively, this integrated analytical strategy provides a pragmatic and scientifically rigorous approach for quality control of DMMJ, while offering a foundational basis for its standardization, rational resource selection, and expanded clinical utilization, thereby supporting its safe and efficacious therapeutic application.