Influence of plant growth regulators and sucrose on micropropagation of potato (Solanum tuberosum) cv. Burin

A study was conducted to evaluate the effects of plant growth regulators, specifically benzyladenine (BAP) and kinetin (KIN), in combination with auxins (IAA and NAA), on the micropropagation of potato (Solanum tuberosum L.) cv. Burin. The experiment used a Completely Randomized Design (CRD), and data were analyzed using ANOVA followed by Tukey’s Honestly Significant Difference (HSD) test (P ≤ 0.05). The results showed that 0.5 mg/L BAP achieved the highest shoot induction rate (70 %), whereas 1.0 mg/L BAP significantly increased the number of branches (3.1 branches/plant). The maximum branch length (3.5 cm) was also observed at 0.5 mg/L BAP. In the multiplication stage, increasing KIN concentration in conjunction with 0.5 mg/L NAA gradually enhanced branch length, reaching a maximum of 13.1 cm at 8.0 mg/L KIN. Regarding microtubers induction, the combination of 80 g/L sucrose with 2.0 mg/L each of BAP and KIN produced the highest number of tubers (ten per jar) and significantly increased average tuber weight to 1.07 g, when compared to the 40 g/L sucrose treatment. These findings show that the synergism between sucrose levels and growth regulators critically governs shoot development and tuberization. Optimal tuber production in cv. Burin is achieved by supplementing the medium with 80 g/L sucrose and 2.0 mg/L BAP + KIN. This study provides a refined protocol for enhancing potato micropropagation efficiency in commercial breeding programs.