Thermally and chemical-thermally modified lysozyme and its bacteriostatic activity

Our investigations indicated a possibility to extend the range of lysozyme activity using thermal and chemical - thermal modification. We found that under the adopted conditions of modification a weakly acid environment (pH 5 - 6) promotes an increased polymerisation of lysozyme and makes it possible to obtain approx. 50% of polymers. It was observed that lysozyme concentration in the solution subjected to thermal modification, the pH value of the solution, the temperature and time of modification have a significant effect on the content of the forming polymers. It was found that the application of lysozyme modification leads to an increase in its antibacterial activity in a comparison to the activity of lysozyme monomer. The modified lysozyme did reduce viability of Gram-positive bacteria and Gramnegative bacteria.