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The structures of aspertetronin A (C16H20O4) and aspertetronin B (C16H22O5), metabolites of a mutant strain of Aspergillus rugulosus, have been elucidated. The use of both high-resolution 1H n.m.r. spectroscopy (220 Mc./sec. and spin decoupling) and ‘element-map’ mass spectrometry have proved determining in establishing that the metabolites are the novel acyltetronic acid derivatives (XXII) and (XXIII) respectively.
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Ballantine et al. (1969) studied this question.
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