A sensitive electrochemical assay method is described for secreted placental alkaline phosphatase (SPAP). The enzyme activity is measured using 2-naphthyl phosphate as the electrochemical substrate. The product of the reaction, 2-naphthol, is detected using Osteryoung square wave voltammetry (OSWV) with good sensitivity and precision using in-house screen printed carbon electrode assembly. The assay format is adapted to develop a cell based assay for herpes simplex virus (HSV).
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Kelso et al. (2000) studied this question.
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