Protein Analysis by SDS‐PAGE and Detection by Coomassie Blue or Silver Staining

Electrophoresis is used to separate complex mixtures of proteins, to investigate subunit compositions and to verify homogeneity of protein samples. It can also serve to purify proteins for use in further applications. In polyacrylamide gel electrophoresis, the combination of gel pore size and protein charge, size, and shape determines the migration rate of the protein. This unit first presents an informative section about safe use of electricity and electrophoresis. Protocols are then provided for one‐dimensional gel electrophoresis under denaturing conditions and subsequent staining with either Coomassie blue or silver staining.Electrophoresis is used to separate complex mixtures of proteins, to investigate subunit compositions and to verify homogeneit