Four isolates of Mycogone perniciosa, separated from diseased culinary-medicinal button mushroom Agaricus bisporus fruiting bodies collected in mushroom farms in Serbia, were studied. The isolates were identified by observing their colony morphology and pathogenic characteristics. Peat/lime casing was the primary source of infection. A sensitivity test for the selected fungicides has shown that all isolates were highly sensitive to iprodione (EC50 = 3.10-4.08 mg L-1), benomyl (EC50 = 0.28-0.46 mg L-1), and, especially, prochloraz-Mn (EC50 = 0,0002-0,008 mg L-1). The minimum inhibitory concentration of prochloraz-Mn was 5 mg L-1, benomyl 10 mg L-1, and iprodione was higher than 1000 mg L-1. The same benomyl concentration had a fungicidal effect, whereas none of the tested iprodione and prochloraz-Mn concentrations were fungicidal for the M perniciosa isolates. Among seven essential oils, Thymus vulgaris oil possessed the highest antifungal activity against the studied mycopathogen, with a minimum inhibitory concentration and minimum fungicidal concentration of 0.02 mu L mL(-1) of air. Pistacia terebinthus essential oil demonstrated the lowest antifungal effect, with minimum inhibitory and minimum fungicidal concentrations of 0.16 and 0.65 mu L mL(-1) of air, respectively.
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Potočnik et al. (2010) studied this question.
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