Diversity ofXanthomonas campestrispv.citrumeloStrains Associated with Epidemics of Citrus Bacterial Spot in Florida Citrus Nurseries: Correlation of Detached Leaf, Monoclonal Antibody, and Restriction Fragment Length Polymorphism Assays

The heterogeneity of 194 previously uncharacterized strains of Xanthomanas campestris pv. citrumelo isolated from citrus bacterial spot epidemics in four citrus nurseries in central Florida was evaluated by virulence reactions on detached leaves, reaction to a panel of monoclonal antibodies (MAbs), and reaction to a panel of restriction fragment length polymorphism (RFLP) probes. Detached-leaf assays were performed on the 194 strains that had been differentiated into five serological groups based on reactions with six MAbs (...)