Fabrication of Biobased Nanocomposites by Chemical Intervention of Nano‐Hydroxyapatite in Aloe Vera Gel‐Guava Seed Matrix for Bone Tissue Engineering

The sharp increase in the bone defect related cases have derived an urgent search for novel bone implants which possess identical mechanical aspect as natural bone, along with commendable osteogenic property. Thus, to accomplish these requirements, in the present study, nano‐hydroxyapatite (nHA) reinforced guava seed (GS) – aloe vera (AV) gel nanocomposites were synthesized via a co precipitation approach with an attempt to induce bone mimicking activity. Three ternary systems were fabricated varying the concentration of different components as – nHA/GS/AV in the ratio of 70/20/10 (HGAV1), 70/15/15 (HGAV2) and 70/10/20 (HGAV3). All of the nanocomposites revealed a high cellular viability confirmed from fluorescence microscopy, increased osteogenic activity, phenomenal antibacterial activity, lower haemolysis, and high protein adsorption. These findings suggested that the use of natural products GS and AV gel for fabrication of nanocomposites gave them profound bioactivity and biocompatibility and exhibited a great potential in dental and orthopaedic applications.