Label-free and real-time detections of the interactions of swine IgG with goat anti-swine IgG by oblique-incidence reflectivity difference technique

With the oblique-incidence reflectivity difference (OIRD) technique, we successfully label-free detected the interaction of swine IgG with different concentrations of 0.125, 0.25, 0.5, 1 mg/ml, and 5 μg/ml goat anti-swine IgG, and real-time detected the reaction dynamic processes of 0.5 mg/ml swine IgG and goat anti-swine IgG with different concentration of 5, 10, and 20 μg/ml, respectively. The interaction times are about 2043, 1828, and 1347 s for the reactions of 0.5 mg/ml swine IgG and goat anti-swine IgG of 5, 10, and 20 μg/ml, respectively. By fitting the reaction dynamic curves, we obtained that the association constant of swine IgG and goat anti-swine IgG is 1620.77 M−1·S−1 at temperature about 22 °C. The experimental results demonstrate that the OIRD is a promising and competing method for label-free and real time detecting the biomolecular interactions and achieving the quantitative information of reaction kinetics.