DNA fingerprinting for individual identification and parentage test in Japanese Black cattle using five different mini-and one micro-satellite probes

We have compared DNA fingerprints (DFPs) in cattle between five different mini- and one microsatellite probes; M13 repetitive sequence, 33.15, YNZ22, α-globin, mo-1 and (GT) n. Within the six probes, M13 and YNZ22 probes gave the more resolvable DFPs in Japanese Black cattle. Using these two probes, we estimated the usefulness for individual identification and parentage test employing two groups of Japanese Black cattle, one being a group of 121 cows of Tajima strain in Mikata area of Hyogo prefecture (the Tajima strain), and the other being a group of 119 carcasses which had participated in the 6th all-Japan Wagyu Show (the JWS carcasses) . All DFPs of the 240 samples revealed individual-specific patterns in both probes. The probability that two randomly selected individuals would have the same DFP are 4.58×10-7 and 6.24×108 for the Tajima strain and for the JWS carcasses, respectively. The probabilities of missing a wrong paternity when the real and putative fathers are unrelated are 6.2×10-3 for the Tajima strain and 1.8×10-3 for the JWS carcasses and when the real and putative fathers are brothers, were 1.2×10-1 for the Tajima strain and 9.4×10-2 for the JWS carcasses. The higher probabilities are usually observed in the Tajima strain than in the JWS carcasses, the reason for which is that DFPs of Tajima strain are less polymorphic than those of the JWS carcasses, caused by the fact that the Tajima strain that higher relationship due to inbreeding.