Cardioprotective and Pulmonary Effects of Intra-lipid on Myocardial Ischemia- Reperfusion Injury in Rat Heart and Lung Tissue

Q: What are the key findings of this study?

Repeated Intralipid administration during early reperfusion significantly reduced myocardial necrosis (7.0% vs 31.5%, P<0.05) and inflammatory infiltration compared to saline control in rats.

Q: What is the clinical evidence from this study?

Population: Myocardial ischemia-reperfusion (I/R) injury and remote pulmonary injury (n=50). Intervention: Intralipid (10% soybean oil-based lipid emulsion) vs. Normal saline (1 mL intraperitoneally at the onset of ischemia). Primary outcome: Myocardial necrosis area (%) in the repeated-dose Intralipid group (Group 4) vs control (p=<0.05).

Q: What does this research mean for the field?

Repeated administration of Intralipid during early reperfusion significantly reduces myocardial necrosis and inflammatory cell infiltration while partially mitigating remote pulmonary injury in rat myocardial ischemia-reperfusion. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.NEUTRAL.

Q: What question did this study set out to answer?

To evaluate the cardioprotective and remote pulmonary effects of Intralipid administered at different time points during myocardial ischemia-reperfusion injury.

Leyla Abdolkarmi; Amir Darbandi-Azar; Farrokh Taftachi; Dina Sadeghi

doi:10.21203/rs.3.rs-10960688/v1

October 14, 2026Open Access

Cardioprotective and Pulmonary Effects of Intra-lipid on Myocardial Ischemia- Reperfusion Injury in Rat Heart and Lung Tissue

View Full Paper

Ask AI

Bookmark

Share

Key result

Repeated Intralipid reduces myocardial necrosis by an absolute ~25% during early reperfusion in rats.

P<0.05
n=50

Why the study?

Intralipid exerts cardioprotective effects against myocardial ischemia-reperfusion injury, but its optimal administration timing and its effects on remote pulmonary injury remained unclear.

Does intraperitoneal administration of a soybean oil-based lipid emulsion at different time points during ischemia-reperfusion reduce histopathological myocardial necrosis, inflammation, and remote pulmonary injury in a rat model of myocardial ischemia-reperfusion?

Key Points

To evaluate the cardioprotective and remote pulmonary effects of Intralipid administered at different time points during myocardial ischemia-reperfusion injury.
Fifty male Wistar rats (300–350 g) were randomly assigned to five groups (n = 10 per group).
Myocardial ischemia-reperfusion injury was induced by 30 minutes of left anterior descending coronary artery occlusion followed by 120 minutes of reperfusion.
Intralipid was administered before or during reperfusion under different timing protocols, followed by histomorphometric evaluation of myocardial necrosis and inflammatory cell infiltration in heart and lung tissues.
All Intralipid-treated groups had significantly less myocardial necrosis and inflammatory cell infiltration than controls (P < 0.05).
Repeated administration (Group 4) provided the greatest protection, reducing myocardial necrosis to 7.0 ± 0.3% versus 31.5 ± 3.4% in controls, and inflammatory cells to 4.3 ± 2.5 versus 29.3 ± 9.4 cells/HPF (P < 0.05 vs. Groups 1, 2, and 3).
Remote pulmonary injury with hemorrhage and inflammatory infiltration occurred following reperfusion; Intralipid reduced this lung damage, although less markedly than myocardial injury.

Structured PICO

Does intraperitoneal administration of a soybean oil-based lipid emulsion at different time points during ischemia-reperfusion reduce histopathological myocardial necrosis, inflammation, and remote pulmonary injury in a rat model of myocardial ischemia-reperfusion?

P

Population

50 healthy young adult male Wistar rats (300-350 g) subjected to 30 minutes of left anterior descending coronary artery occlusion followed by 120 minutes of reperfusion.

I

Intervention

Soybean oil-based lipid emulsion (1 mL of 10% solution) administered intraperitoneally according to four protocols: Group 1 at the onset of ischemia; Group 2 immediately before reperfusion (30 min after LAD ligation); Group 3 at 30 min after reperfusion; Group 4 as a repeated dose at the onset of reperfusion and a second identical dose 30 min later.

C

Comparator

Control group receiving 1 mL normal saline intraperitoneally at the onset of ischemia immediately after LAD ligation.

O

Outcome

Myocardial necrosis area (%) and inflammatory cell infiltration (cells/HPF) in heart tissue at the end of the 120-minute reperfusion period, quantified by histomorphometry on H&E and Masson Trichrome stained sections by a blinded pathologist.surrogate

Main Result

Absolute Event Rate: 7% vs 31.5%

p-value: p=<0.05

Repeated intraperitoneal administration of a soybean oil-based lipid emulsion during early reperfusion significantly reduces histopathological myocardial necrosis and inflammation, while offering milder protection against remote lung injury in a rat model of myocardial ischemia-reperfusion.

Limitations

Conducted exclusively on healthy young male rats without relevant clinical comorbidities (e.g., advanced age, diabetes, hypertension) or concurrent medications.
Brief reperfusion period (2 hours), whereas extending to 24-72 hours might uncover different patterns of protection or injury progression.
Evaluated only histopathology without incorporating biochemical markers (such as troponin and inflammatory cytokines) or functional assessments (such as echocardiography).
Ideal dosage and timing for Intralipid administration to achieve complete multi-organ protection remain unclear.
Conducted exclusively on healthy young male rats without relevant comorbidities (e.g., diabetes, hypertension), older age, or concurrent medications
Brief duration of the reperfusion period (2 hours)
Only evaluated histopathology without incorporating biochemical markers (such as troponin and inflammatory cytokines) or functional assessments (like echocardiography)
Ideal dosage and timing for lipid emulsion administration to achieve multi-organ protection remains unclear

Implication

Hypothesis-generating for Intralipid in reperfusion injury; leaves open translation to human cardioprotection.

Abstract

Objective(s): Myocardial ischemia-reperfusion (I/R) injury is a major contributor to cardiac damage after coronary revascularization. Intralipid, a soybean oil-based lipid emulsion, exerts cardioprotective effects via activation of prosurvival signaling pathways and preservation of mitochondrial function, but its optimal timing and its effects on remote pulmonary injury remain unclear. This study evaluated the cardioprotective and pulmonary effects of Intralipid administered at different time points during myocardial I/R injury in rats. Materials and Methods Fifty male Wistar rats (300–350 g) were randomly assigned to five groups (n = 10/group). Myocardial I/R injury was induced by 30 min occlusion of the left anterior descending artery followed by 120 min of reperfusion. Intralipid was administered before or during reperfusion according to different protocols. Heart and lung tissues were evaluated histopathologically, and myocardial necrosis and inflammatory cell infiltration were quantified by histomorphometry. Results The control group showed extensive myocardial necrosis and inflammatory cell infiltration. All Intralipid-treated groups showed significantly less myocardial injury than controls (P < 0.05). The repeated-dose group showed the greatest protection, with the lowest necrosis (7.0 ± 0.3%) and inflammatory cell infiltration (4.3 ± 2.5 cells/HPF) versus 31.5 ± 3.4% and 29.3 ± 9.4 cells/HPF in controls. Remote pulmonary injury, marked by hemorrhage and inflammatory cell infiltration, was also observed; Intralipid reduced pulmonary damage, but less markedly than myocardial damage. Conclusion Repeated administration of Intralipid during early reperfusion confers significant cardioprotection and may represent an important pharmacological postconditioning strategy for limiting myocardial I/R injury.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Abdolkarmi et al. (2026) studied Myocardial ischemia-reperfusion (I/R) injury and remote pulmonary injury (n=50). Intralipid (10% soybean oil-based lipid emulsion) vs. Normal saline (1 mL intraperitoneally at the onset of ischemia) was evaluated on Myocardial necrosis area (%) in the repeated-dose Intralipid group (Group 4) vs control (p=<0.05). Repeated Intralipid administration during early reperfusion significantly reduced myocardial necrosis (7.0% vs 31.5%, P<0.05) and inflammatory infiltration compared to saline control in rats.

synapsesocial.com/papers/6ae682a3e432e7a5c4ef35e6 https://doi.org/10.21203/rs.3.rs-10960688/v1

View Full Paper

Ask AI

Bookmark

Share

Cardioprotective and Pulmonary Effects of Intra-lipid on Myocardial Ischemia- Reperfusion Injury in Rat Heart and Lung Tissue

Key Points

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Cite This Study

Cardioprotective and Pulmonary Effects of Intra-lipid on Myocardial Ischemia- Reperfusion Injury in Rat Heart and Lung Tissue

Key Points

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading