ImmunoPixel-seqV2 mouse kidney: source selection, tissue preparation and assay v1

ImmunoPixel-seq generates spatial immune-receptor V(D)J libraries and whole-transcriptome libraries from the same mouse kidney section and polony gel. The gel carries immune-receptor constant-region capture probes mixed with poly(T) capture probes. V(D)J products are generated and eluted first. The spatially barcoded cDNAs remain covalently attached to the gel and are subsequently used to prepare WTA libraries by the Pixel-seqV2 second-strand priming method. Mice were prepared by the laboratory of Mariya T. Sweetwyne. The cohort comprises six male mouse kidney records, with four at 23 months and two at 4 months. The source records identify C57BL/6JN mice from NIA. Kidneys were prepared by Mariya Sweetwyne’s laboratory. The paired library identities are listed below.